| Description | Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli |
| Species | Human |
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | A DNA sequence encoding the region (Gly132-Ser288) of human FGF2 |
| Tag | Tag-free |
| Predicted MW | 17.4 kDa |
| Concentration | Resuspend the protein to the desired concentration in proper buffer. |
| Purity | >95% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | Lyophilized from a sterile solution containing 10 mM Tris-Cl, pH 7.0, 150 mM NaCl |
| Endotoxin | <0.1 EU per 1 µg of the protein by the LAL |
| Reference Data |
| Locus ID | 2247 |
| Refseq Size | 6803 |
| Cytogenetics | 4q28.1 |
| Refseq ORF | 864 |
| Synonyms | BFGF; FGF-2; FGFB; HBGF-2 |
| Summary | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton |
