MMAB Mouse Monoclonal Antibody

Carrier-free (BSA/glycerol-free) MMAB mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Clone Name OTI4D12

Applications FC, IF, WB

Recommend Dilution WB 1:2000, IF 1:100, FLOW 1:100

Reactivity  Human, Mouse, Rat

Host  Mouse

Clonality Monoclonal

Immunogen Full length human recombinant protein of human MMAB (NP_443077) produced in HEK293T cell.

Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)

Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Conjugation Unconjugated

Storage Condition Store at -20°C as received.

Predicted Protein Size 24 kDa