VEGFA (NM_001025366) Human Recombinant Protein

Recombinant protein of human Vascular endothelial growth factor A (VEGF) produced in Pichia Pastoris.

Species Human

Expression Host Pichia

Tag Tag-free

Predicted MW 19.2 kDa

Concentration >0.1 µg/µL as determined by microplate BCA method

Purity >95% as determined by SDS-PAGE and Coomassie blue staining

Buffer Lyophilized from a sterile solution containing 20 mM PB, pH 7.2

Note  For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.

Storage Store at -80°C.

Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Bioactivity Measured in a cell proliferation assay using HUVEC Cells. The ED50 for this effect is typically 1.1 - 1.6 ng/ml.

Endotoxin < 0.1 EU per 1 µg of the protein by the LAL