Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli
Species Human
Expression Host E. coli
Concentration >0.1 µg/µL as determined by microplate BCA method
Purity >95% as determined by SDS-PAGE and Coomassie blue staining
Buffer Lyophilized from a sterile solution containing 10 mM Tris-Cl, pH 7.0, 150 mM NaCl
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.07-0.10 ng/ml.
Cell culture factor (PMID: 26253201)
Cell culture factor (PMID: 26704449)
Cell culture factor (PMID: 29416007)
Cell culture factor (PMID: 29937202)
Cell culture factor (PMID: 29948854)