Recombinant protein of human nucleolin (NCL), 20 µg
Species Human
Expression Host HEK293T
Concentration >0.1 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity EMSA reaction positive control (PMID: 26354862)
Taq polymerase assay (regulator) (PMID: 26354862)
Binding assay (Dimethylsulfate footprinting) (PMID: 26354862)
Binding assay (FRET) (PMID: 26354862)
Surface Plasmon Ressonance (SPR) (PMID: 26354862)
Association in cell culture (PMID: 26707270)
Surface Plasmon Ressonance (SPR) (PMID: 27032748)
EMSA reaction positive control (PMID: 27913192)
ELISA binding assay (PMID: 28974366)
